Pocahontas and the Powhatan Dilemma Review Essay

This book was the bases and the most informative piece I have seen on a review of Pocahontas life in my perspective. Pocahontas life is a difficult piece of history to analysis for the fact that her life is only denoted by other persons of history writings. She has no known piece of writing of her own so all knowledge is personal based of outside parties. This makes it for sometimes difficult to truly believe everything for many of the views were from white men during a time period where they had not seen many females weren’t around. I believe the author thesis of the book for the Pocahontas part was her involvement with the English and her growth throughout her life. The other thesis of the book from the Powhatan side was there involvement with the English as well and there plan to use the culture but at the same time push them away. Within the first five chapter of Pocahontas and the Powhatan Dilemma it gives you a base review of history. This first part of the book it explains the culture of the Powhatan and how they are laid out. At this point in the book within the first chapter we find out that Pocahontas is only 9 years of age and her mother is nowhere to be found, but her father is Powhatan the chief of many native people and villages. It talks about the arrival of English in 1603 and we learn that many ships have come before us truly being to talk about John Smith and Jamestown. It also mention the arrival of Don Luis and the Spanish, but doesn’t get into much detail he just seems to pop up with the first few chapters her and there. We learn that Pocahontas name means â€Å"mischief† or the â€Å"little playful one.† The author tells us that the native were not completely different from the Europeans just simply behind the times. We also learn that within the native culture of the Powhatan that there chiefs do not come from the next up son, but from marriage of the daughters. We also learn that the Europeans felt that the women of the tribes need to be saved due to their work in the fields and other jobs around the village. It speaks of the native’s religion of their belief in spirits within everything. John Smith comes into play in 1606 when the English begin to prepare for the Virginia campaign. What I learn from John Smith was that he was a captain that has been captured a lot and has  numerous stories of being saved by women that fall lustful in love with. As kids watching films and stories we are told of Pocahontas is her and John Smith fall in love, but at the time of them meeting Pocahontas would’ve been ten years of age. The plans of the English and John Smith was to: improve native culture, use the land properly, get there before the Spanish, and bring English enlighten, and a place to send the unemployed English. One of the main fears that I read about in the reading was the fear of the English to become like the Indians. The term we learned in class for this is recolonization. After the landing John Smith gets taken by the Powhatan and convince them not to kill him by way of trade. He promises them weapons which out of smarts gives them a cannon which cannot be moved. Both the native and the English realize the need of each. Without one another the English could not survive without food and the natives want weapons or easier put there technology. Over time many English were sent to live with natives to learn their culture and a native would be sent with the English. The most notable was Pocahontas herself was sent with the English to learn their language and lifestyle. The book talks of Pocahontas marriage first to a native warrior, but it fails to mention what happens to him. She later marries John Rolfe an English man who experience much of the reverse colonization. Before her marriage though Pocahontas was kidnapped one of the main characters they looked over her kidnapping was Sir Thomas Dale who was the first Marshall of Virginia. Pocahontas would declare herself a Christian in 1616 and then start her life with John. They would build a log on the land John was granted from the Virginia Company. They would have two children together in their lifetime. Shortly after being settled in there Pocahontas would go to London and experience true English culture. Pocahontas would become very ill around the time the Rolfe family was ready to head back to Virginia. Pocahontas died on March 21, 1617 in the Rolfe inn. What I learned from this book that for someone of her time period and of native culture Pocahontas got to see much more than any Native American of her time period for sure. I learned that much of what we were taught or told of her in are childhood was wrong. I learned that john smith stories  could not be trusted. The feeling I receive as well is Pocahontas rejected her culture and tribe, but in return was probably rejected in a way due to her mother situation. Though she might have be royal in a way just from some of the information I read. The book was good though and I enjoyed the insist of Pocahontas life.

Chromatography for Protein Purification

Department of Chemical & Biomolecular Engineering THE NATIONAL UNIVERSITY of SINGAPORE Chemical Engineering Process Laboratory II Experiment B2 Chromatography for Protein Purification Name Matric No. Group : : : Date of Expt. : GRADE : A. Learning objectives 1. 2. 3. 4. Establish chromatographic assay to determine protein concentrations in a mixture. Appreciate the importance of resolution in protein chromatography. Understand the tension between purity and yield in protein chromatography. Understand the importance of mass balance closure in protein purification.B. Introduction I. Fast Protein Liquid Chromatography (FPLC) High Performance Liquid Chromatography (HPLC) is the workhorse for any biopharmaceutical protein downstream processing train, featuring at least twice within the train. You must recall experiencing the HPLC in one of the experiments in your CN2108 module. Read up on the essential parts of the HPLC system. In this experiment, you will use a modification of the HPLC, the FPLC (Fast Protein Liquid Chromatography System) to separate and purify a mixture of two proteins.The FPLC has been developed to specifically take advantage of the resolution capability of the HPLC for protein purification and collection. II. Concepts in LC When a mixture of proteins is injected into an LC column, the proteins interact with the stationary phase based on their respective chemistries and move through the column at different speed. Based on this differential migration, the proteins elute from the end of the column at different times and therefore become separated. This process is usually facilitated by following the proteins with a mobile phase.Although the protein mixture will have entered as a narrow, concentrated peak, it will exit dispersed and diluted by the mobile phase. This is called bandspreading. Bandspreading (which is an inverse indication of the column efficiency) must be minimized especially for large-scale protein purification. When bandspreading is severe, the proteins may not be sufficiently resolved within a reasonable time-frame. The degree of separation of one component from another is referred to as the resolution (RS), determined based on equation 1 (refer to Fig. 1): RS = VB ? V A 0. (W A + WB ) †¦Eqn. (1) Injection wA VA VB wB Figure 1. Typical protein chromatogram Note that resolution can also be defined based on retention times, instead of volumes. There are various ways to improve resolution, the most straightforward of which is to vary the mode of elution – isocratic versus gradient. Both of these modes are based on the strength of the mobile phase, which directly affects the interactions between the proteins and the stationary phase. In protein chromatography, it is desirable to have high yield as well as high purity of the collected fraction.Yield is the amount of a protein collected as a fraction of the total amount of the same protein fed, while purity is a measure of how much of that protein is in the fraction collected. C. Experimental I. Protein Quantification You will design an experiment to obtain the calibration curves for the two proteins provided using FPLC. You are provided with the following for this experiment: 1. An FPLC system which has been properly set up and equilibrated. You only need to inject 100  µL of each of your samples, and your data will be recorded and analysed by the computer.Note the profile of the mobile phase programmed. 2. A protein mixture containing two proteins (S1 and S2) at concentrations of 1. 0 mg/mL each. II. Protein Purification and Collection You are to perform a chromatographic purification of 1mL of the protein mixture provided. You can expect the chromatogram shown in Fig. 2. Myoglobin Lysozyme Figure 2. Chromatogram of two proteins from FPLC Based on Figure 2, you are to conduct the following: 1. Collect one fraction of the highest yield that is 100% pure S1, and the balance in another fraction. 2.Collect one fraction containing a s much of S1 fed as possible. D. Discussion 1. 2. Briefly describe the experiment that you designed in CI. Explain your choice of the collection times for each of your collection in experiment CII. Determine the yield and purity of each of your collected fractions. Perform a material closure for each of CII (1) and CII (2). Based on your results in (2) above, rationalize the importance of resolution in chromatographic separations. Given the tension between yield and purity, which, in your opinion, is more crucial, yield or purity?How do you propose to improve the resolution of S1 and S2 in this chromatographic purification? 3. 4. Useful Notes 1. Reagents: a. Mixture of two proteins b. Mobile phase – 2M ammonium sulfate in 100mM Phosphate buffer pH 7. 0 c. Elution – 100mM Phosphate buffer pH 7. 0 FPLC to be set up with the appropriate parameters: a. detection wavelength at 280nm b. sample loop – 100  µL c. HIC column for protein separation. d. mobile phase â₠¬â€œ 2 M ammonium sulfate e. mobile phase flow rate: 1 mL/min f. gradient elution – linear gradient 100% to 0% over 10 column volume. 2.

Business analysis of General Electric

Business analysis of General Electric In the history of technology in the united scales, the history of General Electric company constitutes a significant part. The growth of General Electronics (GE) from a mere home laboratory of Thomas Edison to one of the largest companies from simple early applications to complex 21st century high tech wizardry, has been quite outstanding. the company has also merged with other companies, broading its scope , moving from technology to services, consisting of total 11 main operating gates : GE Advanced with high-performance, fused quartz and ceramics which are used by a good number of industries, silicon-based products. One of world’s leading appliance manufacturers, GE Consumer and Industrial is a preeminent global maker of lighting products for consumer, commercial, and industrial customers. Besides, these functions, GE Commercial and Industrial also supplies systems, services and integrated industrial equipment. The energy industry is served by GE Energy, a prominent suppli er among its field. P1. Background to Change Major changes in General Electric: The economic problems of GE can be deciphered through its managerial reshuffling. General Electronic experienced a far reaching change when John F. (Jacx) Welch Jr. became the chairman and CEO in 1981. Welch intended to give power to the subordinates i.e. the periphery of the company by invalidating the hierarchy of the management, assigning divisions according to the per formative skills, which he followed from predecessor Reginald Jone’s time. GE bought 338 business and product lines for $11.1 billion and sold 252 for $5.9 billion over the next few years. Jones wanted GE to be top ranked in all the fields of operation. During this period, in 1943, General Electric Credit Corporation, another of GE’s operations was formed. And as it expanded into other markets like real estate, leasing and selling of heavy industrial goods, insurance and inventories its assets doubled to $16 billion betwee n 1979 and 1984. Also, the leasing operations protected the parent company from heavy taxes. It was done by leasing of the credit corporation on the equipments developed by GE which suffered accelerated depreciation. Forces for change: Controlling bureaucracy: Welch’s signature concept was to spark productivity and save the company from eminent threats. Wales took care of both internal and external problems. He sorted out internal problems such as between functions eg-that between sales and manufacturing and external problems like GE and its suppliers and customers. Welch incited any kind of barrier was bad for the company. Information travels easily in an open, boundary less organization. There is a nothing to hinder the continuous flow of decisions, people, ideas etc. the century-old systems of resided hierarchy and bloated bureaucracy in Ge near got rid of because this Boundary less behavior. Welsh spent two decades reforming the bureaucratic procedures of GE, because he t hought anything that hindered the free flow of ideas and learning was destructive. Lack of sharing information: Because there was a communication gap between the lower and top management, the goals were not clear to them. So they needed to change and adapt so that everyone could be aware of the goals and objectives of the company.

Pttls Essay Example | Topics and Well Written Essays - 1250 words - 1

Pttls - Essay Example I am responsible for training other instructors. I have to ensure that the prospective instructors pass their briefs and assessments. I have to ensure that they acquire the necessary experience and that they have clean records with the R.T.I.T.B. Prospective instructors also have to possess a clean bill of health. This will render them suitable to take up the responsibilities that come with being a reach and counterbalance truck instructor. Qualified instructors determine the quality of the instruction given at the training center. The third major responsibility that I have as an R.T.I.T.B. The instructor is to ensure that the training center is accredited. Accreditation is a continuous process that requires constant adherence. I am required to ensure that all the teaching material is up to date. I also have to ensure that the prospective operators are getting proper instruction. The quality of my work is determined by the kind of operators I am able to train. The team that I set up to assist me in the running the training program will determine the effectiveness of the center. Adherence to the requirements for accreditation will earn the training center a reputation for excellence. Sloppy work and a weak team will result in poor training and the loss of accreditation. It is, therefore, imperative that my team and I pursue excellence in the training program. The R.T.I.T.B. Training protocol is based on three major pieces of legislation. The first is the Health and Safety at work Etc Act of 1974. It requires that the employees of an organization should receive adequate instruction and training. This means that the instruction I give must be thorough and complete. The R.T.I.T.B. Training manuals take this legislation into consideration. This means that I should base my instruction on the R.T.I.T.B. Training manual to reach and counterbalance trucks. The second piece of legislation is the Provision

Narrative of the life of frederick douglass Research Paper

Narrative of the life of frederick douglass - Research Paper Example He highlighted that he had served his entire life as a slave during such meetings. However, his oratory skills made many people doubt that he had served as a slave for more than 20 years. The fact that people doubted him, was one of the factors that pushed him to tell his story by writing a memoir that detailed his entire experience from childhood until the time when he escaped from slavery. Reading the book creates a vivid picture of how it was like to be born into slavery and begin serving as a slave at a very tender age. Notably, Douglass’ memoir was published during a time when the anti-slavery movement was gaining momentum. Therefore, it served to place emphasis on the need to terminate slavery. However, the relevance of the book in the years that would follow declined until the 1960s when the civil rights movement became very active. This paper will discuss the journey taken from Douglass’ text, and how it has emerged as one of the critical literary works in the s lave narrative genre. After Frederick Douglass’ Narrative of the Life of Frederick Douglass, an American Slave was published, it starred an alarming interest on the impacts of slavery. Surprisingly, many people did not realize that slaves were oppressed and mistreated badly. The literature that existed before Douglass’ was published highlighted that slavery benefited both the slave masters and the slaves. The public had been deceived by such information and Douglass wanted to expose the evils of slavery. He was one of the few slaves fortunate enough to exhibit a remarkable level of literacy. His memoir served as a revelation to the real experiences of slaves under their masters. It served to promote the Douglass’ agenda of abolishing slavery. It communicated numerous reasons why African American slaves deserved freedom (Barnes 69). At the end of the memoir, Douglass had stated that he was in pursuit of the freedom of other African American

The Problem of the Identity of Immanuel in Isaiah Essay

The Problem of the Identity of Immanuel in Isaiah - Essay Example Other writers in their interpretations of Isaiah 7:14 have aimed to establish an association of the incident with the birth of Jesus, while, referring to the significance of almah which is a word of Hebrew origin, the meaning of which has been identified as virgin. According to Grisham, the varying assumptions regarding the identity of Immanuel in Isaiah 7:14 have extended to the belief which postulates that he is Jesus himself, while, some consider him to be the child of Isaiah and the king of Judah thus, in their construal of the Book of Isaiah interpreters have vowed to prefer either a single fulfillment or double fulfillment.For the purposes of the succeeding sections of the paper the major views on the identity of Immanuel in Isaiah 7:14 would be assessed in a comprehensive manner. Grisham observes that one of the interpretations regarding the identity of Immanuel in Isaiah 7:14 label the child to be that of Isaiah’s, in his perspective on the identity of the woman and ch ild, Walton notes that in accordance with the teachings of the gospel and that of Christian religion regarding the woman in Isaiah 7:14, much of the assertions point towards the declaration that she is Isaiah’s wife however, these postulations can be nullified on the basis of various arguments. Walton claims his translations of the scripture to be highly credible and reliable. The first argument which nullifies the identity of Immanuel as the child of Isaiah comes from the comparison of 7:14 and 8:3

Impact of Social Media in International Marketing Research Paper

Impact of Social Media in International Marketing - Research Paper Example This essay declares that the emergence of the social media has had a powerful impact on enterprises. In the coming years, social media has the potential to change the landscape of the business environment and the way organizations conduct their business operations. Companies will have to alter the way they conduct business in order to fully take advantage of the opportunities presented by the emergence of social media. Companies that quickly understand the changes brought about by social media on customer value and customer engagement will be in a good position to realize optimum benefits from the use of social media. This paper stresses that the emergence of new technologies, such as the worldwide web, mobile devices and digital television has created a number of opportunities for businesses to produce and market innovative goods and services. It has also helped customers to get increased value for their money. Traditional marketing approaches limit the number of customers a company can reach to hundreds, thousands or even tens of thousands. These marketing techniques also require a lot of time, effort and cost. In sharp contrast, a business today through the use of computers and the Internet can reach millions of customers with minimal effort and cost. China has gone beyond the United States in terms of the leading country that has the maximum number of Internet users. Asia Pacific will have more than 500 million of the Internet population. In terms of the world Internet population, the percentage of users in the Latin American and South Pacific regions will increase and that of North America an d Europe will decrease even though the actual numbers will continue to increase (Luukka). Social media allows

Product Lunch Presentation Essay Example | Topics and Well Written Essays - 1000 words

Product Lunch Presentation - Essay Example However, these advancements had their equal share of success and failure. They were also associated with severe criticism; criticism which resulted from fatal or minor errors committed by practitioners, which have proven to be detrimental to a patient’s life. In addition to that, the scope of these advancements was only limited to carrying out basic medical procedures and even after that they came at a great cost. This deficiency in the field of medical practices combined with the desire of human being to have a healthy and convenient lifestyle influenced researchers and scientists all over the world to come up with an innovative idea. That is when the concept of robotics was introduced into health care system all over the world just like it is used in the field of construction, manufacturing and information technology. Robotics in health care system also witnessed a rapid advancement since the time of its inception. But there were disadvantages associated with this technology that came in the form of huge size, high cost of production and maintenance. Thus the idea of making it portable and cost effective came into limelight and thus efforts were fostered in to achieving this goal. Till now various portable health robots have been developed which can perform basic level medical procedures but some disadvantages associated with them are that they are not user friendly and that their functionality is limited to a certain extent. This is where a new innovation called â€Å"MY HEALTHY ROBOT† developed by me/my company fits into the health care system thereby addressing the deficiencies noted in this line of operation. MY HEALTHY ROBOT – WHAT IS IT? â€Å"My healthy robot† is a user friendly portable health care device which provides an in depth analysis of a client’s health thereby giving easy to read description of the client’s health status as output. Since the output can be easily analyzed by anybody, hence it disqualif ies the need for visiting a doctor to get the results analyzed. It is an advanced device which provides the client with a painless procedure (no needle method) to examine blood sugar level. This process is called the â€Å"Swab Saliva Process† which would only require the client to swab saliva and stick it inside the device and wait for a few minutes and thereafter the robot will dispense the health report. This will offer the client with a convenient method of examining his blood without having to wait for countless of hours in the doctor’s chamber and that too in empty stomach. It displays accurate results within a span of few minutes and hence it is less time consuming. The product also has an additional feature called the â€Å"My baby mode† which will be helpful for pregnant women or anyone taking care of a baby. â€Å"MY HEALTHY ROBOT – PRICING OPTION & FACILITIES† According to Allen & Dare (2004), price of a product or service is a critical factor which attracts and engages customers with the attribute of the product or service. Similar theory has been explained by Anderson & Simester (2003). Product price in general can influence the purchasing decision of a customer (Bizer & Petty, 2002; Coulter, 2001). It can be explained by the fact that buying the product will depend upon the purchasing power of the customer. In light of these theories, efforts have been made to keep the price of the product at an appropriate level in order make the product available to

Development of Health Care Essay Example | Topics and Well Written Essays - 1000 words

Development of Health Care - Essay Example In Elizabethan times, parishes had to bear with the responsibility of taking care of the poor living in the community (Liverpool Echo 2009). The poor in those days could benefit from "outdoor relief" in the form of food, clothing and fuel. However, with an unwieldy appreciation in the poor population, the parishes decided to withdraw this already insufficient outdoor relief and started resorting to "indoor relief" in the guise of hell like workhouses that required the less miserable amongst the poor lot of Britain to bear with inhuman work conditions in lieu of a pitiable yet indispensable sustenance (Liverpool Echo 2009). However, the things got worst at the start of the 19th century, which led to the introduction of Poor Law Amendment Act of 1834. Zillions of workhouses came up all across the UK. Poor Relief reduced to a shameful pittance accessible only to those who were willing to opt for exploitative workhouses instead of a hapless existence. The life in such workhouses was far from being bearable and homely. Comfort was an elusive pie in the sky that was far beyond the reach of even the relatively well to do poor. In fact, the possibility of affiliating to some workhouse was a perpetual fear that haunted the deprived families huddled in the slums to bear with their hopeless existence. Workhouse records and Census returns do elaborately testify to the miserable lot of the poor in the 19th century. Workhouses were a dreaded place divested of all hope and future. The moving spirit behind these workhouses was to keep the living standards of the inmates much below that of the lowest paid labourer. The poor had to bear with subhuman rules and norms, which made their lives frustratingly monotonous (Hull Daily Mail 2007). They ha d to carry their pitiable status with them in the guise of grey tweed suits and blue and white striped dresses (Hull Daily Mail 2007). A harsh policy of gender segregation went to the extent of separating even married couples and siblings.Homelessness and abject poverty appropriated within their scope a plethora of health problems. The life expectancy amongst the workhouse residents was very low and they exhibited some of the highest death rates in the UK. The poor huddled in the workhouses were four times more prone to accidents and thirty five times more likely to commit suicide (Herald Express 2008). Chest and skin infections, gastrointestinal troubles, poor circulation, compromised dental hygiene, anaemia, and visual and nutritional problems perpetually defined the life at a workhouse (Herald Express 2008).Yet, the biggest eliminator tended to be the extreme mental and emotional turmoil that the poor in Britain had to cope up with in these workhouses. The senseless and absurd pr actice of classifying the poor by the criteria of 'deserving poor' and the 'undeserving poor' added insult to injury. The 'deserving' being those who were too old or infirm to work while the 'undeserving' being wandering beggars and destitute. The principle of less eligibility smacked of a festering annoyance and intolerability in the society and the state that intended to keep the unemployed deliberately poor; and vulnerable to or rather forced to taking jobs for which they were essentially unqualified or averse to (Baggot 2004). Of course, such